Marcelo Bielsa's 24-man list also included fellow striker Edinson Cavani but he has withdrawn after a muscle injury in Boca Juniors' 1-0 win over Newell's Old Boys on Sunday.
Mexican club Leon's Federico Vinas has therefore been drafted in as a late replacement.
The 36-year-old Suarez, who played his last game for Uruguay in the 2-0 win over Ghana in December at the World Cup, has made 137 international appearances, scoring 68 goals.
The striker could return to the top of the all-time South American World Cup qualifying scoring charts, where he trails Argentina's Lionel Messi by two goals with a total of 31.
Another record within reach is the number of games played for Uruguay in World Cup qualifiers, currently held by Diego Godin with 65 appearances, three more than the Gremio forward.
Persons:
Uruguay's Luis Suarez, Edinson Cavani, Kai Pfaffenbach, Luis Suarez, Cavani, Marcelo Bielsa's, Leon's Federico Vinas, Suarez, Lionel Messi, Diego Godin, Sergio Rochet, Franco, Santiago Mele, Ronald Araujo, Jose Maria Gimenez, Bruno Mendez, Sebastian Caceres, Matias Vina, Sassuolo, Guillermo Varela, Mathias Olivera, Manuel Ugarte, Rodrigo Bentancur, Felipe Carballo, Nicolas de la, Federico Valverde, Giorgian de Arrascaeta, Agustin Canobbio, Maximiliano Araujo, Facundo Pellistri, Facundo Torres, Cristian Olivera, Darwin Nunez, Federico Vinas, Angelica Medina, Ken Ferris
Organizations:
Soccer Football, FIFA World, Qatar, Lusail, Edinson Cavani REUTERS, Boca Juniors, Newell's, Sunday, South, Argentina, Ghana, Gremio, Sporting, Atletico Madrid, Flamengo, Paris St, Tottenham Hotspur, Athletico Paranaense, Manchester United, Strikers, Angeles FC, Thomson
Locations:
Portugal, Uruguay, Lusail, Qatar, Bolivia, Internacional, Franco Israel, Barcelona, America, Paris, Paris St Germain, Nicolas de la Cruz, Real Madrid, Athletico, Toluca, Manchester, Orlando City, Angeles, Liverpool, Leon, Mexico City